real-time taqman reverse transcription pcr  (MultiTarget Pharmaceuticals)

Journal: Vaccine: X

Article Title: Effectiveness of the severe acute respiratory syndrome coronavirus 2 Omicron BA.5 bivalent vaccine on symptoms in healthcare workers with BA.5 infection

doi: 10.1016/j.jvacx.2024.100433

Figure Lengend Snippet: Trend of infection in healthcare workers The bar graphs show the number of healthcare workers (n = 531) infected each week during the observation period. ( A ) Trend by infection route obtained through phone interviews. ( B ) Trend by variant classified using the TaqMan assay.

Article Snippet: The diagnostic tests used were TaqMan real-time reverse transcription-polymerase chain reaction (PCR) targeting the nucleocapsid gene in the StepOne plus real-time PCR system (Thermo Fisher Scientific, Waltham, MA, USA) according to the protocol developed by the National Institute of Infectious Diseases in Japan , , the FilmArray Respiratory Panel 2.1 test in the FilmArray Torch system (bioMérieux, Marcy-l’Etoile, France) , the Xpert Xpress SARS-CoV-2 test in the Cepheid GeneXpert system (Cepheid, Sunnyvale, CA) , cobas SARS-CoV-2 & influenza A/B in the cobas Liat system (Roche Diagnostics, Basel, Switzerland), and the Lumipulse antigen test in the LUMIPULSE G600II system (Fujirebio, Inc., Tokyo, Japan) , .

Techniques: Infection, Variant Assay, TaqMan Assay

Journal: Microbial Cell

Article Title: Detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and its first variants in fourplex real-time quantitative reverse transcription-PCR assays

doi: 10.15698/mic2022.01.767

Figure Lengend Snippet: Oligonucleotide primers and fluorogenic probes used in real-time quantitative RT-PCR assays.

Article Snippet: In this study, we present a multitarget real-time TaqMan reverse transcription PCR (rRT-PCR) assay for the quantitative detection of SARS-CoV-2 and some of its circulating variants harboring mutations that give the virus a selective advantage.

Techniques: Quantitative RT-PCR, Sequencing

Journal: Microbial Cell

Article Title: Detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and its first variants in fourplex real-time quantitative reverse transcription-PCR assays

doi: 10.15698/mic2022.01.767

Figure Lengend Snippet: Limits of detection of SARS-CoV-2-specific primers and probes using synthetic RNA transcripts in singleplex rRT-PCR assays.

Article Snippet: In this study, we present a multitarget real-time TaqMan reverse transcription PCR (rRT-PCR) assay for the quantitative detection of SARS-CoV-2 and some of its circulating variants harboring mutations that give the virus a selective advantage.

Techniques:

Journal: Microbial Cell

Article Title: Detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and its first variants in fourplex real-time quantitative reverse transcription-PCR assays

doi: 10.15698/mic2022.01.767

Figure Lengend Snippet: Sensitivities of SARS-CoV-2-specific primers and probes in fourplex rRT-PCR assays.

Article Snippet: In this study, we present a multitarget real-time TaqMan reverse transcription PCR (rRT-PCR) assay for the quantitative detection of SARS-CoV-2 and some of its circulating variants harboring mutations that give the virus a selective advantage.

Techniques:

Journal: Microbial Cell

Article Title: Detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and its first variants in fourplex real-time quantitative reverse transcription-PCR assays

doi: 10.15698/mic2022.01.767

Figure Lengend Snippet: Specificities of fluorogenic SARS-CoV-2 probes with a number of other viruses and bacteria.

Article Snippet: In this study, we present a multitarget real-time TaqMan reverse transcription PCR (rRT-PCR) assay for the quantitative detection of SARS-CoV-2 and some of its circulating variants harboring mutations that give the virus a selective advantage.

Techniques: Bacteria, Virus